FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n02_DJA247_DOP2_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n02_DJA247_DOP2_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35542856
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGAT52300.012.0023521
ACATCTA245150.010.559474
CGCGGAT30100.010.2693021
CGCCGAT58150.09.97709751
CCGGAAT96350.09.9699671
GCGGAAT56500.09.9317921
ATCTACA246600.09.5729086
CGGCAAT58900.09.4463641
CGAATGT77450.09.3222843
CGGGAAT82850.09.1837821
TTCGATT60850.09.1366322
AATCGAG47550.09.0900945
CATCTAC255000.09.0898595
CTCGAAT90100.08.5503581
GGAAAAT341950.08.4415111
GTCGGTT74950.08.4386711
GGGAAAT240950.08.3287471
CCGGTAT33200.08.3077611
CCCACAT296300.08.2013121
CGGAAAT93200.08.163911
Produced by FastQC (version 0.11.5)