FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18951110
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG265781514.024587477989417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA257650.030.8458942
GATCGGA257750.029.906541
CGGAAGA268900.029.5356754
TCGGAAG269500.029.2183843
AGAGCAC269850.029.06138
CGTCTGA135450.028.12617316-17
GAGCACA285400.027.6772029
ACGTCTG141850.026.9575414-15
ACACGTC147000.025.8188312-13
CACGTCT149050.025.44830314-15
CACACGT160300.023.9876112-13
TCCCTAC65900.023.122919
CGCGAAA20350.023.09425436-37
TCTCGTA59550.023.03790342-43
CTCGTAT56400.022.89409644-45
ATGCCGT69800.022.24589348-49
AAGAGCA367900.021.9352767
GCACACG178250.021.43900710-11
TATCTCG28250.021.25645840-41
GAAGAGC380600.020.9039846
Produced by FastQC (version 0.11.5)