FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n01_FDK263_LDI4_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n01_FDK263_LDI4_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38458555
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT1300260.338093825938078TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG218900.038.99307648-49
CTCGTAT203650.037.63307644-45
TCGTATG238250.035.64673244-45
CGTATGC249200.034.41395646-47
TCTCGTA209600.034.219342-43
CGGCTAT258050.033.21536634-35
ATGCCGT265250.032.29578848-49
CCGGCTA278750.030.79140932-33
TCACCGG280250.030.57571630-31
ATCTCGT226750.030.53139942-43
TGCCGTC283850.030.10420250-51
ACCGGCT288000.029.81069432-33
GCCGTCT288050.029.40966650-51
CCGTCTT287600.029.38134652-53
GTATGCC296400.028.99783346-47
GATCTCG240000.028.9547240-41
GTCACCG312500.027.710728-29
CACCGGC321300.026.79495430-31
TATGATC281300.025.2270938-39
CGTCTGA354450.024.91922616-17
Produced by FastQC (version 0.11.5)