FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n01_FDK261_LP6_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n01_FDK261_LP6_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22058911
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT565380.2563045836668909TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG101800.036.90744848-49
CTCGTAT95150.035.1938444-45
TCGTATG112500.033.33388544-45
TCTCGTA97150.032.36696642-43
CGTATGC117700.032.14364646-47
CGGCTAT120750.031.03667834-35
ATGCCGT129650.029.08930448-49
ATCTCGT106150.028.6830242-43
CCGGCTA132150.028.50318132-33
TCACCGG134350.028.0010530-31
TGCCGTC137550.027.14221850-51
ACCGGCT139900.027.0939632-33
GCCGTCT140050.026.40334150-51
CCGTCTT138050.026.39014852-53
GATCTCG119300.025.78019140-41
GTATGCC150450.025.38339646-47
GTCACCG151800.024.8462428-29
CACCGGC160400.023.61637130-31
TATGATC138400.022.6170638-39
CGTCTGA180900.021.22903416-17
Produced by FastQC (version 0.11.5)