FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n01_FDK256_LDI2_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n01_FDK256_LDI2_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29698824
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT1132260.38124741908972554TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG171000.039.29094748-49
CTCGTAT167750.038.1409844-45
TATCTCG162550.036.87736540-41
TCGTATG184750.036.4825144-45
CGTATGC192350.035.38669646-47
TCTCGTA176800.034.28124642-43
ATGCCGT205600.032.8173748-49
ATCTCGT188950.032.00145342-43
TGCCGTC221700.030.32682250-51
CCGTCTT218650.030.06553852-53
GCCGTCT223850.029.4838550-51
GTATGCC235900.028.8740246-47
AGCTATC242050.025.33428638-39
GCTATCT238800.025.27131538-39
CTATCTC247950.024.71229740-41
CGTCTGA283950.024.62385416-17
ATCGGAA569750.024.1971472
GAGCACA592700.023.9226939
CGTCTTC278700.023.68121352-53
CGGAAGA588600.023.6325954
Produced by FastQC (version 0.11.5)