FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n01_FDK255_LP5_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n01_FDK255_LP5_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18681155
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT239600.12825759435109874TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG79350.038.22084434-35
AATGCGC87400.034.4831234-35
TATGCCG48400.031.74796148-49
ATGCGCA106200.028.24458336-37
ATCGCGT17300.028.00538642-43
TGCGCAT100750.027.76876436-37
CTCGTAT48900.026.90657444-45
CGCGTAT16200.026.53512244-45
CGTATGC56150.026.5201146-47
TCGCGTA15350.026.45728342-43
GCGCATC103600.025.56062338-39
CTAATGC123650.024.73896832-33
ACTAATG125850.024.6461932-33
TCGTATG52150.024.09122544-45
TCTCGTA54500.023.92397542-43
CGTCTGA140750.022.03737416-17
GTCACTA140700.021.9281628-29
CACTAAT146750.021.23320630-31
CGCATCG41750.020.87689438-39
CGCATCT87750.020.73184838-39
Produced by FastQC (version 0.11.5)