FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n01_FDK254_LP3_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n01_FDK254_LP3_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19716794
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT570630.2894131774161661TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG89700.036.82919748-49
TCGTATC92900.036.7365738-39
TATCTCG87900.035.61099240-41
CTCGTAT89150.035.1116844-45
TTCGTAT104800.034.7406936-37
ACGAATT114600.034.17432-33
AATTCGT112850.034.07223534-35
CGAATTC115250.033.79579532-33
CGTATCT96100.033.28907838-39
GAATTCG118250.033.0786534-35
TCTCGTA93350.032.66692442-43
CGTATGC102450.032.08349646-47
ATTCGTA119500.032.05708336-37
TCGTATG101150.031.62709244-45
GTCACGA125700.030.89353228-29
CACGAAT127850.030.55799730-31
ATGCCGT110600.029.5475148-49
AGTCACG133900.029.19673728-29
ATCTCGT106350.029.0980942-43
TCACGAA137200.028.61399730-31
Produced by FastQC (version 0.11.5)