FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n01_FDK239_LP4_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n01_FDK239_LP4_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18426524
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT495680.2690035299115558TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG80800.037.68187748-49
CTCGTAT82200.035.2198744-45
TCGTATG87850.034.49568644-45
TATCTCG79550.033.9748840-41
CGTATGC90550.033.86052346-47
TCTCGTA85850.031.89663542-43
ATCTCGT95400.028.82810442-43
ATGCCGT108850.027.94966348-49
TGCCGTC113700.026.7991850-51
GCCGTCT114300.025.99359750-51
CCGTCTT115300.025.78871752-53
GTATGCC121400.025.43200346-47
CGTCTGA144600.021.97616816-17
AGCTATC128350.021.92695838-39
CTATCTC128050.021.60738640-41
GCTATCT128700.021.33217638-39
ATCGGAA305250.020.464872
ACGTCTG155300.020.41640514-15
ACACGTC156200.020.07071912-13
GATCGGA313300.019.878661
Produced by FastQC (version 0.11.5)