FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n01_FDK237_LDI3_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n01_FDK237_LDI3_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31850461
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT567090.17804765839966963TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG178600.039.0419534-35
AATGCGC188150.036.94667434-35
TATGCCG101800.035.01787648-49
ATGCGCA217950.031.64443236-37
CTCGTAT100450.031.46917344-45
TGCGCAT202700.031.2483336-37
ATCGCGT35700.030.13622942-43
GCGCATC206750.028.6260538-39
TCGCGTA31400.028.43918442-43
ACTAATG253100.027.96292932-33
TCTCGTA112700.027.5429642-43
CTAATGC258700.027.2015632-33
CGTATGC128350.027.1080746-47
CGCGTAT34000.027.03268844-45
TCGTATG113200.026.87570844-45
GTCACTA277500.025.53108428-29
ATCTCGT134500.025.09173642-43
CGCATCT171050.024.58976638-39
CGTCTGA290500.024.55097816-17
ATGCCGT136350.024.0544548-49
Produced by FastQC (version 0.11.5)