FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n01_FDK218_LDI1_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n01_FDK218_LDI1_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22955455
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT285070.12418399025416835TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA95750.040.52948436-37
GCGAAAT106250.036.01010536-37
CCGCGAA112850.035.18779434-35
TCCGCGA114250.034.96448534-35
TATGCCG56350.030.85127348-49
CGAAATC108000.029.81904638-39
ACTCCGC139450.028.9355832-33
AATCGCG24000.028.30170440-41
CACTCCG148700.027.42313830-31
CTCCGCG154600.026.2843932-33
CTCGTAT57500.025.73215344-45
AATCTCG76200.025.49506640-41
CGTATGC69300.023.92093346-47
TCGTATG62650.023.54108244-45
ATCGCGT21200.023.30152542-43
TCTCGTA68650.022.07176242-43
CGCGTAT16800.021.91181244-45
CGTCTGA193100.021.6465516-17
TCGCGTA17500.021.171142-43
ATCTCGT83400.020.5887142-43
Produced by FastQC (version 0.11.5)