FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n01_FDK216_LP2_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n01_FDK216_LP2_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14851455
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT324370.21840957670477404TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG56750.034.4003448-49
TCGTATC59250.034.3517838-39
TTCGTAT66300.033.2064936-37
CTCGTAT54250.033.0524744-45
AATTCGT70550.032.99004434-35
CGTATGC61100.031.95121646-47
ACGAATT72850.031.85095832-33
TATCTCG57500.031.7625440-41
TCGTATG59050.031.65276144-45
ATTCGTA72950.031.31890336-37
CGTATCT61150.031.07064438-39
CGAATTC75850.030.71644232-33
GAATTCG75900.030.60207734-35
CACGAAT78650.029.80434430-31
TCTCGTA62500.027.9675642-43
GTCACGA85550.027.37415328-29
AGTCACG88350.026.34531228-29
TCACGAA89500.026.08504730-31
ATCTCGT68900.025.81780642-43
ATGCCGT76550.025.44048948-49
Produced by FastQC (version 0.11.5)