FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n01_DJA270_DOM2_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n01_DJA270_DOM2_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33617462
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT873590.2598619729234765TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG140450.037.2518948-49
TCGTATG149350.034.69810544-45
TATCTCG133300.034.5109340-41
CTCGTAT142600.034.17543844-45
CGTATGC156450.033.6395446-47
TCTCGTA152200.030.55309542-43
ATGCCGT190650.027.2064248-49
ATCTCGT173000.026.72865342-43
TGCCGTC203050.025.6150650-51
GCCGTCT204600.024.91026550-51
CCGTCTT207700.024.61849452-53
GTATGCC216050.024.39264746-47
AGCTATC219300.021.71368638-39
CGTCTGA260200.020.81077816-17
GCTATCT225750.020.6198838-39
CTATCTC230300.020.4290340-41
ATCGGAA557900.019.2102872
CGTCTTC272550.018.91766752-53
ACGTCTG285600.018.91014914-15
AAGCTAT269750.018.82363336-37
Produced by FastQC (version 0.11.5)