FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n01_DJA269_DOM3_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n01_DJA269_DOM3_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31282667
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT502160.16052339782921962TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG162000.038.61498334-35
AATGCGC177250.035.3462734-35
TATGCCG96400.033.3084248-49
TGCGCAT192350.029.87979136-37
ATGCGCA206950.029.83748836-37
CTCGTAT93950.029.02018444-45
TCGCGTA29100.028.97284142-43
ATCGCGT36650.028.2532442-43
CGCGTAT31000.028.19299144-45
GCGCATC195150.027.74738538-39
CGTATGC112700.026.7629646-47
ACTAATG240750.026.67446932-33
TCGTATG96300.026.43769344-45
TCTCGTA100100.026.3119642-43
CTAATGC242150.026.02986132-33
GTCACTA265100.024.20818528-29
ATCTCGT123600.024.03779242-43
CGTCTGA274850.023.49496316-17
CGCATCT166850.022.57525638-39
ATGCCGT127450.022.5103148-49
Produced by FastQC (version 0.11.5)