FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n01_DJA268_DODI2_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n01_DJA268_DODI2_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24269752
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT576230.23742723040598027TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG104250.036.7234148-49
CTCGTAT98950.035.0661844-45
TCGTATG117750.032.33156244-45
CGTATGC124250.031.09891546-47
TCTCGTA104250.031.0281642-43
CGGCTAT128600.030.08396334-35
ATGCCGT137000.028.27401448-49
CCGGCTA139500.028.02279732-33
TCACCGG138350.027.94667830-31
ATCTCGT118550.026.9648842-43
ACCGGCT150250.025.9071932-33
TGCCGTC150150.025.73453350-51
GCCGTCT149150.025.52491850-51
CCGTCTT148200.025.46418452-53
GTATGCC154000.025.21454446-47
GATCTCG129700.024.44534940-41
GTCACCG162450.023.87585828-29
CACCGGC173150.022.79625330-31
TATGATC149750.021.69575138-39
CGTCTGA186200.021.23697716-17
Produced by FastQC (version 0.11.5)