FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n01_DJA267_DODI3_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n01_DJA267_DODI3_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26551903
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT272290.10255008840609277TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA91500.040.36138536-37
GCGAAAT105550.034.35876536-37
CCGCGAA112450.033.39085434-35
TCCGCGA114400.033.23689734-35
CGAAATC108550.028.07072438-39
AATCGCG22800.027.49956740-41
TATGCCG59500.027.3819248-49
ACTCCGC142150.026.83224932-33
CACTCCG154200.024.98186330-31
CTCCGCG158150.024.34289232-33
CTCGTAT61350.024.31094644-45
TCGTATG61200.023.43917344-45
CGTATGC67750.023.24133146-47
AATCTCG79650.023.16818640-41
ATCGCGT20400.022.70184742-43
CGCGTAT17450.020.95955544-45
TCGCGTA17800.020.8142842-43
CGTCTGA194250.020.16127816-17
TCTCGTA76250.019.529242-43
ACGTCTG208550.018.76766814-15
Produced by FastQC (version 0.11.5)