FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n01_DJA263_DOP3_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n01_DJA263_DOP3_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24841746
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT809340.325798355719441TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG130650.038.1192348-49
CTCGTAT127150.036.6655844-45
TATCTCG118700.036.6547540-41
TCGTATG135100.036.26590344-45
CGTATGC141750.035.10068546-47
TCTCGTA133650.033.1055142-43
ATCTCGT146000.030.48408142-43
ATGCCGT163400.030.3773148-49
TGCCGTC176200.028.2648550-51
CCGTCTT175450.027.93894852-53
GCCGTCT174100.027.8282350-51
GTATGCC181200.027.45873346-47
AGCTATC185300.024.2237838-39
CTATCTC185000.024.09617240-41
CGTCTGA212800.024.09567616-17
ATCGGAA422800.023.9444472
GCTATCT185950.023.74317238-39
GATCGGA429450.023.3859881
CGGAAGA443950.023.0399744
GAGCACA454650.022.8807779
Produced by FastQC (version 0.11.5)