FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n01_DJA260_DODI1_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n01_DJA260_DODI1_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23489810
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT280730.11951139664390645TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG95900.036.57781634-35
AATGCGC109350.031.81813834-35
TATGCCG60550.030.98633848-49
ATGCGCA130900.026.38055436-37
CTCGTAT61900.026.16682444-45
CGTATGC68400.026.11073346-47
TGCGCAT124100.026.06540736-37
ATCGCGT19900.025.77857642-43
TCGCGTA18000.025.06913642-43
TCGTATG62350.024.94951844-45
GCGCATC128100.023.91661638-39
CGCGTAT20100.023.74965544-45
ACTAATG153000.023.4548532-33
CTAATGC153700.023.06989132-33
TCTCGTA74200.022.24534642-43
CACTAAT178300.020.33980830-31
CGTCTGA178900.020.21851316-17
GTCACTA179000.019.98257428-29
ATCTCGT90250.019.9471242-43
CGCATCT113550.019.84898238-39
Produced by FastQC (version 0.11.5)