FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n01_DJA258_DOP1_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n01_DJA258_DOP1_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27669589
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT764160.27617323842432207TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG132400.038.02807248-49
CTCGTAT124800.035.43414344-45
TCGTATG145250.034.09155344-45
CGTATGC155050.032.73325746-47
CGGCTAT159550.032.00357434-35
TCTCGTA131100.031.84741642-43
ATGCCGT163450.030.41170348-49
CCGGCTA172650.029.58926832-33
TCACCGG175100.029.24317730-31
ATCTCGT140700.028.88111342-43
TGCCGTC176850.028.3758550-51
CCGTCTT178900.027.55948652-53
ACCGGCT188750.027.24151832-33
GCCGTCT181400.027.06186550-51
GTATGCC190850.026.68019946-47
GATCTCG153650.026.3851640-41
GTCACCG198600.025.72420328-29
CACCGGC209000.024.52263530-31
TATGATC180200.023.11711338-39
CGTCTGA226550.023.10520716-17
Produced by FastQC (version 0.11.5)