FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n01_DJA248_DOM1_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n01_DJA248_DOM1_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33146360
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT430440.12986041302876095TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA140350.041.76270336-37
GCGAAAT158200.036.40500636-37
CCGCGAA167500.035.6172534-35
TCCGCGA173000.034.8281134-35
CGAAATC158900.030.78932638-39
TATGCCG86950.030.34594348-49
AATCGCG33550.029.87285640-41
ACTCCGC201600.029.86388232-33
CACTCCG219000.027.6430230-31
CTCCGCG223800.027.10314232-33
CTCGTAT86750.026.50096944-45
AATCTCG111600.026.19694940-41
CGTATGC98550.025.49673546-47
TCGTATG91950.025.0539344-45
ATCGCGT28700.024.57714542-43
CGCGTAT24150.024.09377144-45
TCTCGTA100350.023.52479442-43
TCGCGTA25600.022.91468242-43
CGTCTGA280950.022.19022416-17
ACGTCTG297400.020.89906314-15
Produced by FastQC (version 0.11.5)