FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n01_DJA247_DOP2_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n01_DJA247_DOP2_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35542856
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT512530.1442005673376388TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA167450.041.41470736-37
GCGAAAT185050.036.76989736-37
CCGCGAA191850.036.60550734-35
TCCGCGA196200.036.03601534-35
AATCGCG39400.033.63531540-41
TATGCCG95000.032.79947348-49
ACTCCGC227500.031.22437532-33
CGAAATC193550.029.92789338-39
CACTCCG245850.029.04846430-31
CTCCGCG246800.028.88845332-33
CTCGTAT92950.028.33605644-45
ATCGCGT35650.028.17986742-43
AATCTCG121250.027.08931240-41
TCGCGTA28750.026.18662542-43
TCTCGTA109750.024.21495642-43
TCGTATG111800.023.36727544-45
CGTCTGA316450.023.0854416-17
CGTATGC129850.022.60650446-47
GAGCACA672400.022.0763959
ATCTCGT135250.021.91469442-43
Produced by FastQC (version 0.11.5)