FastQCFastQC Report
Wed 18 Nov 2020
HHGJGAFX2_n02_ADT1_1106.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGJGAFX2_n02_ADT1_1106.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169987528
Sequences flagged as poor quality0
Sequence length116
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTC28829950.0109.338421
CGTCCCT28820800.0109.1915054
CCCGTCC28881850.0109.04992
TCTGCCA41092750.0108.890169
CCGTCCC28875350.0108.8867653
GTCCCTA28966000.0108.796595
TTCCTCA11375300.0108.513642
CATATCC16910950.0108.4045946
GTCTGCC41432000.0108.034848
CCTCATT11972350.0107.982244
TGGCCGA13035050.0107.976876
TTAGAGT41862850.0107.764733
GAGTCTG41747450.0107.717316
AGTCTGC41692950.0107.693627
ATATCCC16951650.0107.647217
CTTGGCC14462600.0107.51634
ACTGCCA17016450.0107.509231
GGCCGAA13083950.0107.4158867
AGTCACC20686050.0107.350045
ATTCCTC11513950.0107.321851
Produced by FastQC (version 0.11.5)