FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9016402
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4017254.4554912258792365No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC584580.6483517482916135Illumina Single End PCR Primer 1 (100% over 50bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCAGATCGGAAGAGCGTCGTGTA191860.21278997986114637Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GTGACTGGAGTTCAGACGTGTGCTCAGATCGGAAGAGCGTCGTGTAGGGA184520.20464926031470201Illumina Single End PCR Primer 1 (100% over 25bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCGAGATCGGAAGAGCGTCGTGT142030.1575240323135548Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GTGACTGGAGTTCAGACGTGTGCTAGATCGGAAGAGCGTCGTGTAGGGAA122090.13540878057566644Illumina Single End PCR Primer 1 (100% over 26bp)
GTGACTGGAGTTCAGACGTGTGCTCTTAGATCGGAAGAGCGTCGTGTAGG109250.12116806681867114Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GTGACTGGAGTTCAGACGTGTGCTCTAGATCGGAAGAGCGTCGTGTAGGG103730.11504589081099091Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCAGATCGGAAGAGCGTCGTGTAG96990.10757062517842482Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTGA497800.067.7572862
ACTGAAG488100.067.0045244
GTGACTG274500.066.3634341
TGACTGG273200.066.282212
GATCATC211350.066.09959410
CTGAAGA520350.065.963825
GGAGTTC259900.065.603617
ACTGGAG276250.065.554864
ATCATCC185050.064.9729611
TGAAGAT548900.064.7845156
ACGTGTG244100.064.7772616
TCCAGTA143800.064.6657815
AGATCAT242050.064.592379
CGTGTGC238900.064.5029417
AGTTCAG262250.064.3387159
TCAGACG251150.064.2378112
GAGTTCA266450.064.186128
ATCGTCC58150.064.0107811
TGGAGTT290800.063.9584776
GTGTGCT234200.063.6909518
Produced by FastQC (version 0.11.5)