FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n02_DS2297-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n02_DS2297-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6861573
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC619120.9023003908870458Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG183500.26743138927473337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA138950.038.85963447
CCGTATC135300.038.20121849
TCGCCGT140950.038.08479746
AAAGGGG112750.038.0528766
GTCGCCG144750.037.64101845
GGTCGCC145950.037.6195344
GCCGTAT146800.037.06770348
AGGGGGG116800.036.7927968
CGTATCA142850.036.2801750
AAGGGGG118150.036.07619567
CTCGGTG138600.035.80306238
GTGGTCG153300.035.74815442
ATCTCGG140400.035.26927636
TCTCGGT142050.034.7364237
TGGTCGC158050.034.6071143
GATCTCG141500.034.4757335
GGTGGTC161750.033.7940941
GTATCAT148100.033.576651
TCGGTGG158350.032.8624639
AGATCTC152800.032.3839734
Produced by FastQC (version 0.11.5)