FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n02_DS2296-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n02_DS2296-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7794491
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3907195.012758369982081Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG634550.8141006256855001No Hit
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC114270.14660354345139406Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC85590.10980832487971312Illumina Single End PCR Primer 1 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG69850.053.8384781
TGACTGG70450.052.7772182
GACTGGA75800.051.160773
AAGGGGG538400.049.49619767
AAAGGGG539850.049.22253866
AGGGGGG554700.048.83065868
CCGTATC673050.046.10287549
GTATCAT654900.045.71370751
CGCCGTA699650.045.59580247
CGTATCA683100.045.5731650
TCGCCGT703150.045.095746
ACTGGAG81650.045.0401424
GCCGTAT710500.044.97310648
CATTAAA699900.044.6159655
AAAAGGG601050.044.39692765
ATCATTA694300.044.1797153
GTCGCCG733500.044.15057445
GGTCGCC744850.044.08858544
TATCATT688900.043.9773852
TCATTAA703150.043.7381354
Produced by FastQC (version 0.11.5)