FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n02_DS2294-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n02_DS2294-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6115436
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC678081.1088007461773781Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG171610.28061776789095655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA134150.041.45231647
CCGTATC132100.041.06140549
AAGGGGG110600.040.5950367
AGGGGGG112450.040.4562268
AAAGGGG112750.040.06956566
GGTCGCC143550.039.9321544
GCCGTAT141100.039.90626548
TCGCCGT138750.039.90148546
GTCGCCG142400.039.49279845
CGTATCA139050.039.16007250
CTCGGTG137300.038.6409538
GTGGTCG148350.038.42811642
ATCTCGG139250.038.04988536
TCTCGGT140050.037.70759637
GATCTCG140350.037.2280335
TGGTCGC154300.036.9462843
TCGGTGG151800.036.3559539
GTATCAT144600.036.20467851
GGTGGTC159950.035.8159541
AGATCTC148250.035.3852734
Produced by FastQC (version 0.11.5)