FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n02_DS2293-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n02_DS2293-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7413307
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC93413312.60075968795033Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1407231.898248649354465No Hit
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC322400.4348936311419451Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC238500.3217187686952665Illumina Single End PCR Primer 1 (100% over 40bp)
GTGACTGGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT158520.21383169481582243Illumina Single End PCR Primer 1 (100% over 39bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC108620.1465203046359742Illumina Single End PCR Primer 1 (98% over 50bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC102990.13892585319884904Illumina Single End PCR Primer 1 (98% over 50bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC92120.12426303133001237Illumina Single End PCR Primer 1 (100% over 49bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC85750.11567037490825618Illumina Single End PCR Primer 1 (100% over 49bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC82090.11073330701129738Illumina Single End PCR Primer 1 (100% over 49bp)
AGAGCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC77760.10489245892555105Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG75100.10130431668349901Illumina Single End PCR Primer 1 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTGG158450.063.8654142
GTGACTG161550.063.7808231
GACTGGA176100.059.3528863
ACTGGAG175050.056.9568024
CTGGAGT155650.052.3247955
GAGTTAG94100.051.527928
AGTTAGA96100.050.491639
AAGGGGG1212700.049.64427667
AGGGGGG1262350.049.29862668
AAAGGGG1233700.048.6086366
AGATAGA120900.048.41777
GATCGAG41250.048.03698
CATTAAA1564750.047.96862455
ATCATTA1545600.047.6372153
TCATTAA1554850.047.55863654
GGAGTTA102850.047.5198827
GTATCAT1522150.047.38024551
TATCATT1539900.047.1564452
CCGTATC1587500.046.48980749
CGTATCA1601250.046.39000350
Produced by FastQC (version 0.11.5)