FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n02_DS2261-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n02_DS2261-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9685527
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2294822.36932899985721Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG526370.5434603610108154No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG57450.048.903491
AGGGGGG340200.044.41832768
AAAGGGG340150.044.1790866
GACTGGA67650.043.3895383
AAGGGGG345800.043.37582867
TGACTGG65100.043.0071532
CGCCGTA441200.042.69753347
CCGTATC427400.042.33167349
TCGCCGT444950.042.24373646
GCCGTAT455550.041.57532548
GGTCGCC467200.041.5725144
GTCGCCG457450.041.55564545
CGTATCA442500.041.0769450
GTGGTCG478850.040.5466842
CTCGGTG438900.040.53783838
GTATCAT435050.040.24416751
ATCTCGG440950.040.1904436
TCTCGGT442500.039.9622537
GATCTCG439500.039.84527235
TGGTCGC486550.039.8256843
Produced by FastQC (version 0.11.5)