FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n02_DS2260-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n02_DS2260-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8137363
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC5375776.6062801917525364Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1072131.3175398467537949No Hit
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC221960.27276649695976446Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC144650.17776028917476092Illumina Single End PCR Primer 1 (100% over 40bp)
GTGACTGGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT98970.12162416743606007Illumina Single End PCR Primer 1 (100% over 39bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC85320.10484969147867682Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG106700.059.5645331
TGACTGG110100.057.1629332
GACTGGA117750.054.709323
ACTGGAG119150.052.057694
AAGGGGG715100.051.25642867
AAAGGGG719600.050.8596566
AGGGGGG733400.050.8509768
CTGGAGT103250.050.2615285
AAAAGGG788800.046.6240965
GAGTTAG74100.044.364628
CCGTATC965450.044.2685349
CATTAAA980350.044.22477755
GTATCAT944750.044.1497451
GATCGAG31400.044.040978
CGTATCA975850.044.0083250
CGCCGTA998050.043.80108347
ATCATTA974400.043.7732153
TCATTAA982350.043.5077554
TCGCCGT1002250.043.43596646
TATCATT974350.043.23337652
Produced by FastQC (version 0.11.5)