FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n02_DS2259-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n02_DS2259-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5666008
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC767811.3551163358752758Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG183910.32458478703171617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG128450.042.55626366
CGCCGTA159600.041.50823647
AGGGGGG133950.041.3822168
CCGTATC153700.041.16514249
AAGGGGG134500.040.7446667
TCGCCGT163400.040.5218746
GGTCGCC170150.040.1274844
GTCGCCG166700.040.0972345
GCCGTAT165050.040.0948648
CGTATCA159150.039.84342250
CTCGGTG163700.038.6952838
GTGGTCG177200.038.51157442
ATCTCGG164300.038.2557636
GTGACTG26900.038.082861
GTATCAT162150.037.89768251
TCTCGGT166250.037.76494637
TGACTGG27500.037.5914422
GATCTCG165850.037.45509735
TGGTCGC182400.037.3557743
GGTGGTC184150.037.07744241