FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n02_DS2258-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n02_DS2258-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6998578
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC445770.6369436762725228Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG190620.27236961565620904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA91050.047.27346847
CCGTATC90800.046.44079649
GGTCGCC103950.046.3235444
TCGCCGT91400.046.0983146
GCCGTAT101100.045.10125748
GTCGCCG107550.044.83771545
ATCTCGG107800.044.7012736
CTCGGTG110100.043.95847738
TCTCGGT111300.042.88651737
GATCTCG112900.042.34103435
CGTATCA104100.042.2218650
GTGGTCG116450.042.1026642
TGGTCGC109000.041.6410143
AGATCTC118400.039.7534934
TCGGTGG133850.039.58303539
GGTGGTC123800.039.5747641
TAGATCT125700.038.2797433
GTAGGGA146950.037.6049619
CGGTGGT138750.037.580140
GTATCAT115500.036.9943451