FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n02_DS2228-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n02_DS2228-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6980928
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC847661.2142511711909936Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG216450.310059063780632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA184900.041.54311447
CCGTATC180600.041.35054449
AAAGGGG149200.041.071566
AGGGGGG151750.040.70332768
TCGCCGT188550.040.6281446
GTCGCCG193250.040.03841845
GGTCGCC196400.039.8773544
CGTATCA187600.039.86386550
GCCGTAT194850.039.72703648
AAGGGGG154700.039.49762367
GTGGTCG203500.038.41726342
CTCGGTG190100.037.81195438
TGGTCGC206850.037.69357743
ATCTCGG190850.037.64529436
GTATCAT192650.037.4017551
TCTCGGT194150.036.8967537
GATCTCG191050.036.8731835
GGTGGTC213250.036.71002241
TCGGTGG206800.035.9768839
GTGACTG29350.035.728481
Produced by FastQC (version 0.11.5)