FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n02_DS2227-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n02_DS2227-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7651447
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1667352.1791303004516664Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG306320.40034257572456555No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG51150.047.4512631
TGACTGG53500.044.9300352
AAAGGGG245600.043.9014366
GACTGGA57550.043.8848153
AGGGGGG251200.043.42284868
AAGGGGG249750.043.24086467
CCGTATC302200.043.0663949
CGCCGTA310900.042.94183347
TCGCCGT312300.042.48040446
CGTATCA308900.042.22319850
GCCGTAT322900.041.86591748
GTCGCCG324450.041.48309345
GGTCGCC330450.041.46060644
GTATCAT310000.040.26742651
TGGTCGC339550.040.17450743
GTGGTCG341400.040.05931542
CTCGGTG317500.039.72430838
ATCTCGG318650.039.3832636
TCTCGGT321200.038.9613937
GATCTCG319600.038.806335