FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n02_DS2226-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n02_DS2226-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7973892
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1705402.138729744521245Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG296440.3717632493643004No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG49200.046.35081
AAAGGGG248400.045.67479766
AGGGGGG251650.045.3199168
GACTGGA53850.044.695163
AAGGGGG254850.044.4350367
TGACTGG52050.043.9702762
CCGTATC320400.041.75390249
CGCCGTA333300.041.6825347
TCGCCGT337650.041.042446
GGTCGCC352100.040.7691144
CGTATCA330600.040.6244450
GTCGCCG346050.040.5818645
GCCGTAT344250.040.48832348
GTATCAT327650.039.52702751
GTGGTCG362350.039.49104342
TGGTCGC364500.039.2096143
CTCGGTG337700.038.9329438
ATCTCGG338650.038.6170536
TCTCGGT342750.038.11450637
GGTGGTC376350.038.01222641
Produced by FastQC (version 0.11.5)