FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n02_DS2225-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n02_DS2225-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6965174
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1710322.455530902745574Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG273590.39279707872337427No Hit
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC81240.11663743073755228Illumina Single End PCR Primer 1 (100% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG58850.052.439581
TGACTGG61500.049.96472
GACTGGA67100.047.6028753
ACTGGAG70800.043.7732164
CCGTATC320700.043.60453849
CTGGAGT66200.043.425325
CGCCGTA328450.043.19393547
TCGCCGT329800.042.51901646
CGTATCA332100.042.381750
GCCGTAT340800.042.09044348
GTATCAT333350.041.3097551
AGGGGGG269850.040.91554668
GTCGCCG360400.040.8995145
GGTCGCC361700.040.86861844
GGAGTTC58900.040.8351177
AAAGGGG250500.039.92692666
TGGTCGC365250.039.7336443
GTGGTCG370500.039.6617842
CATTAAA365100.039.57714555
AAGGGGG258250.039.2572267
Produced by FastQC (version 0.11.5)