FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n02_DS2223-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n02_DS2223-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9220147
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC533410.578526567960359Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG226200.24533231411603307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA107600.040.78397447
TCGCCGT110950.039.61541746
GGTCGCC115150.039.5070244
GTCGCCG115250.038.86588745
CCGTATC110450.038.52719549
GTGGTCG120850.037.70195442
GCCGTAT116300.037.64348
CTCGGTG113500.037.21538
ATCTCGG116000.036.35261536
AAAGGGG108250.035.78669766
TCTCGGT118150.035.57262437
GATCTCG120000.034.7907235
CGTATCA122850.034.66670650
TGGTCGC130950.034.6600643
GGTGGTC131900.034.6228641
TCGGTGG129400.034.34599339
AGGGGGG115450.034.1906768
CGGTGGT141300.031.89854840
AGATCTC132050.031.85394934
AAGGGGG121600.031.77078467