FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n02_DS2222-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n02_DS2222-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7056132
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1601982.2703373462968095Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG463750.6572297683773489No Hit
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC91960.13032636010777576Illumina Single End PCR Primer 1 (100% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG49300.052.432051
TGACTGG53250.047.9056932
GACTGGA58850.046.3075033
AGGGGGG257400.044.21243368
AAGGGGG256800.043.89391367
AAAGGGG267500.042.1913866
ACTGGAG65500.040.1006974
CTGGAGT62050.037.4697885
GATCGAG20000.037.4594768
AAAAGGG349100.032.5797965
GCACACG11800.032.3288318
CCGTATC419150.031.5259449
CGTATCA427350.031.00268750
CGCCGTA441800.030.92444447
GAGTTAG47850.030.8751038
TCGCCGT445800.030.49781846
GTATCAT417450.030.41341851
GCCGTAT453550.030.1923148
GTCGCCG461450.029.97877145
GGTCGCC470950.029.59738344
Produced by FastQC (version 0.11.5)