FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n02_DS2221-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n02_DS2221-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6752037
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4007135.934697928936112Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG604380.8951076541790278No Hit
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC168900.25014673349686917Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC132830.1967258177050866Illumina Single End PCR Primer 1 (100% over 40bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC107980.15992210943156857Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT71850.10641233156749585Illumina Single End PCR Primer 1 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG105650.061.823531
TGACTGG107200.060.4754372
GACTGGA116000.057.1922573
ACTGGAG119000.053.7086564
CTGGAGT112450.050.4102825
AAGGGGG528500.048.755467
AAAGGGG528900.048.69386366
AGGGGGG535000.048.4766768
GGAGTTC95750.045.810017
AAAAGGG578200.044.8449765
TGGAGTT150650.044.0133746
CATTAAA733000.043.3846355
GATCGAG26700.043.1349688
CCGTATC744450.042.83767749
GTATCAT715400.042.74802851
CGTATCA749800.042.6210150
AGATAGA104600.042.5030487
ATCATTA737350.042.3642353
TCATTAA741300.042.20427754
CGCCGTA786800.042.03679347