Basic Statistics
Measure | Value |
---|---|
Filename | HHGHNAFX2_n02_DS2221-1.dk.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 6752037 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 400713 | 5.934697928936112 | Illumina Single End PCR Primer 1 (100% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 60438 | 0.8951076541790278 | No Hit |
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC | 16890 | 0.25014673349686917 | Illumina Single End PCR Primer 1 (100% over 38bp) |
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC | 13283 | 0.1967258177050866 | Illumina Single End PCR Primer 1 (100% over 40bp) |
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC | 10798 | 0.15992210943156857 | Illumina Single End PCR Primer 1 (98% over 50bp) |
GTGACTGGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT | 7185 | 0.10641233156749585 | Illumina Single End PCR Primer 1 (100% over 39bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTGACTG | 10565 | 0.0 | 61.82353 | 1 |
TGACTGG | 10720 | 0.0 | 60.475437 | 2 |
GACTGGA | 11600 | 0.0 | 57.192257 | 3 |
ACTGGAG | 11900 | 0.0 | 53.708656 | 4 |
CTGGAGT | 11245 | 0.0 | 50.410282 | 5 |
AAGGGGG | 52850 | 0.0 | 48.7554 | 67 |
AAAGGGG | 52890 | 0.0 | 48.693863 | 66 |
AGGGGGG | 53500 | 0.0 | 48.47667 | 68 |
GGAGTTC | 9575 | 0.0 | 45.81001 | 7 |
AAAAGGG | 57820 | 0.0 | 44.84497 | 65 |
TGGAGTT | 15065 | 0.0 | 44.013374 | 6 |
CATTAAA | 73300 | 0.0 | 43.38463 | 55 |
GATCGAG | 2670 | 0.0 | 43.134968 | 8 |
CCGTATC | 74445 | 0.0 | 42.837677 | 49 |
GTATCAT | 71540 | 0.0 | 42.748028 | 51 |
CGTATCA | 74980 | 0.0 | 42.62101 | 50 |
AGATAGA | 10460 | 0.0 | 42.503048 | 7 |
ATCATTA | 73735 | 0.0 | 42.36423 | 53 |
TCATTAA | 74130 | 0.0 | 42.204277 | 54 |
CGCCGTA | 78680 | 0.0 | 42.036793 | 47 |