FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n02_DS2220-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n02_DS2220-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6547531
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1002551.5311878630280635Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG251830.38461826297576907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA195300.043.36341547
GTGACTG34150.042.831381
CCGTATC196150.042.56890549
TCGCCGT197800.042.12534346
GCCGTAT206650.041.26962348
GGTCGCC213450.041.0530344
CGTATCA206050.040.965250
GTCGCCG213250.040.8785145
GTGGTCG218100.039.87350542
CTCGGTG214300.039.71565638
TGGTCGC217350.039.43085543
TGACTGG36800.039.3281442
GTATCAT210200.039.22439651
ATCTCGG219900.039.0060336
TCTCGGT216850.038.90942437
GATCTCG222600.038.26564835
GACTGGA39700.038.1149373
GGTGGTC228500.037.85988641
AGGGGGG181550.037.70332368
TCGGTGG232600.037.4335539