FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n02_DS2219-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n02_DS2219-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6396295
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC805351.2590882690682652Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG397800.6219225348424362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG147750.038.1564568
GTGACTG29450.037.3978921
AAGGGGG146500.036.35635467
TGACTGG30650.035.7858852
CGCCGTA204900.033.74854347
CCGTATC205450.033.28346349
GACTGGA35050.033.2798043
TCGCCGT205150.033.22952346
CGTATCA212850.032.58642250
GCCGTAT216850.032.1951348
GGTCGCC223750.032.1876344
GTCGCCG221850.032.1478145
GTGGTCG227550.031.49680942
CTCGGTG222700.031.47585738
TGGTCGC225750.031.40690843
ATCTCGG224500.031.36378536
TCTCGGT223950.031.15953637
GATCTCG224850.031.05013535
GTATCAT217450.030.96414851
AAAGGGG170350.030.7538866
Produced by FastQC (version 0.11.5)