FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n02_DS2218-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n02_DS2218-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3771455
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC878422.329127617855708Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG149370.3960540428031092No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG37100.056.2589231
TGACTGG39000.053.8270872
GACTGGA42600.050.1646083
CTGGAGT39850.048.0700765
ACTGGAG45500.046.2645154
AGGGGGG130700.044.12340568
GGAGTTC37350.043.2115027
AAGGGGG133200.042.9537467
AAAGGGG136550.041.97738666
CCGTATC176350.039.54909549
CGCCGTA186800.039.52849647
CGTATCA178800.039.14417650
TCGCCGT189000.038.90070346
TGGAGTT57250.038.4057466
GCCGTAT192000.038.366848
GTCGCCG195350.038.15569345
ACGTGTG25700.037.99560516
GGTCGCC202350.037.82200244
GTATCAT180000.037.46397851
TGGTCGC205450.036.94471743
Produced by FastQC (version 0.11.5)