FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9016402
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA4234084.695975179456284TruSeq Adapter, Index 14 (97% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG363430.40307652653464204No Hit
GAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAAC259140.28740954540403146TruSeq Adapter, Index 14 (96% over 30bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCT235970.261711933429765TruSeq Adapter, Index 14 (97% over 34bp)
AGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACT186410.2067454401434186TruSeq Adapter, Index 14 (96% over 29bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTC172160.19094090968880933TruSeq Adapter, Index 14 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCACTCACAGATCGGAAGAGCACA166220.18435291594141434TruSeq Adapter, Index 13 (97% over 35bp)
AAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGA156770.17387201679783135TruSeq Adapter, Index 14 (96% over 32bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAT149040.1652987522073661TruSeq Adapter, Index 2 (97% over 37bp)
AGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAA142100.15760166860350724TruSeq Adapter, Index 14 (96% over 31bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTG132250.14667713351733871TruSeq Adapter, Index 14 (96% over 33bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCG112850.12516079030194086TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGCCACAGATCGGAAGAGCACA111130.12325315574882309TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCAGTCACAGATCGGAAGAGCACAC101930.11304952906935604Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC96900.10747080709134309TruSeq Adapter, Index 14 (97% over 36bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGT95610.10604008117650478TruSeq Adapter, Index 14 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGGA32500.044.704760
GTCGGAG10800.040.7182122
CACAGTG108550.038.5384168
CAGTGAG19000.038.28404670
ATGCCGG21950.038.16280748
ACAGTGA108500.038.00307569
GGTGGAA38850.037.95139361
AAAAAGG108300.037.47611670
CTTAGGC45600.036.81925268
TGCCGGG21750.036.7392949
CACGTGG84250.036.64149566
TCACGTG109050.036.5953665
ACTTAGG46000.036.5031667
CCGATGT48600.036.2810168
AATCGGA26350.036.1742741
TTAGGCA45750.036.1584869
GTGGAAA60900.036.087662
ACCGATG49400.036.05299867
CACCGAT50000.035.72253466
CCGGGGT19200.035.5840651
Produced by FastQC (version 0.11.5)