FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n01_DS2297-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n01_DS2297-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6861573
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATG680800.9921923150857683TruSeq Adapter, Index 21 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTG18450.036.0420158
GATCTCG148350.034.65660540
CGATCTC152600.033.5996639
CTCGTAT151950.033.09846543
TATGCCG162050.032.72015847
GCGTGAA32750.032.70106560
TCGATCT159550.032.28960838
ATCTCGT159450.032.13426241
ATGCCGT160400.032.05303648
GTATGCC166650.031.98546
TGCCGTC149400.031.97671349
TCGGCGT21700.031.45043457
TCTCGTA161600.030.84042742
GCGTGGA17250.030.83942460
GCCGTCT119450.030.6769650
GGCGTGA16950.030.55932859
AGTCGGA13750.030.3136311
TTCGATC177350.029.99605637
CGTATGC180200.029.52164845
CCGTCTT128700.028.6352951
Produced by FastQC (version 0.11.5)