FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n01_DS2296-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n01_DS2296-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7794491
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGGGGGTG995561.27726108093524TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGGGGTTG369380.4738988087868727TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGCGGATG268870.34494875932244967TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGGGGATG237780.30506161338822513TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGGGGGTT228710.29342518966280157TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGCGGTTG187080.2400156726077431TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGCGTATG158500.2033487497772465TruSeq Adapter, Index 20 (97% over 41bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGG146080.1874144187221462TruSeq Adapter, Index 20 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGTATG136700.1753802781990511TruSeq Adapter, Index 20 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGGATG126310.16205035068999374TruSeq Adapter, Index 20 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGCGGGTG120380.15444241323775984TruSeq Adapter, Index 20 (97% over 41bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCC112490.14431987925831205TruSeq Adapter, Index 20 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATG102340.13129786152809722TruSeq Adapter, Index 20 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGGGGGTG99980.1282700820361458TruSeq Adapter, Index 20 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGGGGTTT89370.11465790389648278TruSeq Adapter, Index 20 (97% over 41bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGC81740.10486893884411438TruSeq Adapter, Index 20 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGGA202350.057.30209460
ATGCCGG74200.055.75269748
GCGTGGA67250.055.3214160
GGTGGAA214900.054.46056761
GTGGAAA285250.054.2679762
AAAAAGG420300.051.0366970
CCGGCGT43150.050.93673751
AGTCGGA30750.050.4604261
GGTGGCG149600.049.71410847
GGATGCC52950.049.70546746
TGCCGGC50850.049.6245549
GATGCCG46550.049.62225747
GGGCGTG66050.049.17308458
GTGGCGG156750.048.20558548
CGGGGTG286350.048.08276452
GCCGGCG42500.047.9276750
GGCGGAT45050.047.7785143
TGGGCGT69700.047.04994257
TGGAAAA340100.046.93596363
CCGGGGT87450.046.74501851
Produced by FastQC (version 0.11.5)