FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n01_DS2294-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n01_DS2294-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6115436
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGGGGGTG181500.2967899590478913TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGGGGTTG75140.12286940783944106TruSeq Adapter, Index 19 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGGA46750.046.71518760
TGGCGGG51550.043.18008849
ATGCCGG21300.042.5574248
CGGGGTG63950.041.53902452
TGGGGGT55200.040.7053157
GCGGGGT47100.040.42342451
GGCGGGG55200.040.00786650
GTGGGGG68800.039.7807956
CGGGGGT44100.038.3321943
GCGTGGA21150.038.22581560
GTGGGCG20150.038.0385756
ATCGGGG77800.037.7430941
GGGCGTG20000.037.44888358
GGTGGAA59450.037.3243761
TCGGGGA15000.037.09889642
GGGTGGG75400.036.90208454
GGGTGGC37000.036.32324246
GGTGGGG68450.036.25166355
TGCCGGG23650.035.96087349
CGGGGAT15350.035.7969843
Produced by FastQC (version 0.11.5)