FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n01_DS2293-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n01_DS2293-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7413307
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGGGTG2095372.826498349522015TruSeq Adapter, Index 18 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATG1163541.5695289565102324TruSeq Adapter, Index 18 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGCGTATG1081721.4591598594257598TruSeq Adapter, Index 18 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGCGGATG743481.0028992459100912TruSeq Adapter, Index 18 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGGTTG697390.9407272624754378TruSeq Adapter, Index 18 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGGATG668830.9022019457713002TruSeq Adapter, Index 18 (97% over 42bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCC419010.5652133386624889TruSeq Adapter, Index 18 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGGGTT408100.5504965597674559TruSeq Adapter, Index 18 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGTATG346140.46691712618943204TruSeq Adapter, Index 18 (97% over 42bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGC323430.43628302456649914TruSeq Adapter, Index 18 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGCGGTTG322380.43486665262884705TruSeq Adapter, Index 18 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGCGTTTG288870.3896641539329209TruSeq Adapter, Index 18 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGCGGGTG285670.38534759183721917TruSeq Adapter, Index 18 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGTTTG251330.33902548484772044TruSeq Adapter, Index 18 (97% over 42bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCG231780.312653988294293TruSeq Adapter, Index 18 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGTGTG212110.28612062066227667TruSeq Adapter, Index 18 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGGATG201830.272253664929835TruSeq Adapter, Index 18 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG149790.20205557384848624No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTGGTATG142180.19179024961464566TruSeq Adapter, Index 18 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTTTG135300.18250964110888704TruSeq Adapter, Index 18 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGGTTT121980.16454195138552874TruSeq Adapter, Index 18 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTGGGATG101800.13732063166950997TruSeq Adapter, Index 18 (97% over 45bp)
TCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCA97390.1313718695313711TruSeq Adapter, Index 18 (100% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGGATT94940.12806700167684948TruSeq Adapter, Index 18 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGCGTATT90760.12242849243933915TruSeq Adapter, Index 18 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGCGTGTG89570.12082327091000007TruSeq Adapter, Index 18 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTGGGGTG77860.10502735149104173TruSeq Adapter, Index 18 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGCGGATT74680.10073776790843816TruSeq Adapter, Index 18 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA68650.060.816991
GTGGAAA729300.060.18839362
GGTGGAA500900.059.47476661
GCGTGGA236100.058.59802660
GGGTGGA506000.058.35656460
TGGAAAA786850.057.88121863
ATGCCGG261900.057.2354148
AAAAAGG916950.057.1956970
CGTGGAA245700.057.10617461
GATGCCG124000.055.82860647
GGATGCC141150.054.22756246
CGCGGAT114300.053.8300543
TCGCGGA116900.053.3812842
GTCGGAA74100.053.0602152
GGGCGTG231900.052.95839758
TGGGCGT236750.052.65757
TATGCCG297100.052.1859147
CGGATGC96900.052.08272645
CCGGGGT259450.051.79999551
GTATGCC317250.051.05566446
Produced by FastQC (version 0.11.5)