FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n01_DS2261-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n01_DS2261-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9685527
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATG1893391.9548652334560628TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCGCGTATG132950.1372666660265363TruSeq Adapter, Index 3 (97% over 37bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAG116820.12061295167521602TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTGTG114880.11860996309235418TruSeq Adapter, Index 3 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGGA77600.048.3038460
GGGTGGA68700.046.8179860
GGGCGTG64700.042.73431458
TCGGCGT79300.042.5900457
GTCGGCG53750.042.51954756
GGCGTGA68000.042.2045159
GTGGGGG76550.041.97120356
AGTCGGA28000.041.7805671
GGGGGTG74750.041.62406558
GCGTGAA117800.041.4163160
CGTCGGC55050.041.32472255
AAAAAGG313500.041.1836770
TGGGGGT78550.040.76888757
GTGGGCG57000.040.647856
GCCGGGG37100.040.47038350
GCCGTGG61850.040.17654450
GTGGAAA176350.040.1489662
GCCGTCG126800.039.77394550
TGCCGTG79750.039.76046449
TGGGCGT71500.039.6490657
Produced by FastQC (version 0.11.5)