FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n01_DS2260-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n01_DS2260-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8137363
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATG3004963.692793353325887TruSeq Adapter, Index 13 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCGCGTATG325430.3999207114147421TruSeq Adapter, Index 13 (97% over 42bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTC278670.34245737839150103TruSeq Adapter, Index 13 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTGTG241400.2966563000814883TruSeq Adapter, Index 13 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGGATG222680.2736513044827913TruSeq Adapter, Index 13 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCGCGGGTG205890.25301808460554115TruSeq Adapter, Index 13 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTTTG203460.25003185921532567TruSeq Adapter, Index 13 (97% over 46bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAA187690.2306521166623635TruSeq Adapter, Index 13 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCGCGGATG161230.19813544019112828TruSeq Adapter, Index 13 (97% over 42bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCA138400.1700796683151532TruSeq Adapter, Index 13 (100% over 38bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC112350.13806684057230825TruSeq Adapter, Index 13 (97% over 48bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86130.10584509994208198No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCGCGGTTG82210.10102781453893601TruSeq Adapter, Index 13 (97% over 42bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA48750.057.6206051
GGGTGGA213650.055.6146660
GCGTGGA169600.054.7474460
GTGGAAA391500.054.74643362
GGTGGAA226800.053.03821661
CGTGGAA181650.051.61664261
CGGGGGT52550.051.54904643
AAAAAGG608150.050.23497470
TGGAAAA457100.049.99054363
TCGGGGG54650.049.95245442
ATCGGGG71050.049.21005241
GGGCGTG164800.049.057758
GGAAAAA524050.048.51938664
GTCGGAA55200.047.8873062
GTGGGCG160050.047.78022456
CCGGGGT133800.047.10966551
GCGGGGT153050.046.9470251
TGGGCGT179600.046.78840657
GCCGTGG97100.046.42469450
GTCGGCG79300.046.38546856
Produced by FastQC (version 0.11.5)