FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n01_DS2259-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n01_DS2259-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5666008
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG177590.3134305493391467TruSeq Adapter, Index 12 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATG58620.10345908442063619TruSeq Adapter, Index 12 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGGA50650.049.68168360
GCGTGGA29200.044.46700760
GGGCGTG28300.044.0261258
CGGGGTG68700.042.99644552
ATGCCGG30050.042.97627348
GTGGGGG69150.042.7166456
GGCGGGG47050.042.548450
GCGGGGT46300.041.80141451
GTGGGCG29300.041.20959556
GGTGGAA63000.040.4980261
GTGGAAA95850.040.0919462
TGGGGGT64900.039.905657
GGGTGGG77000.039.8616954
GCCGGGG28150.039.78491650
GGTGGGG68900.039.56991255
TGGGCGT32250.039.17638457
TGCCGGG30650.039.0519349
AATCGCG27550.038.99991240
TATGCCG57200.038.17995547
AGTCGGA14900.037.8536951
Produced by FastQC (version 0.11.5)