FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n01_DS2258-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n01_DS2258-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6998578
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG645260.9219872951333828TruSeq Adapter, Index 11 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGGA31400.042.0210560
GGCGTGA25400.041.4751559
GGGCGTG28100.039.98102658
GGGTGGA29700.039.2412660
GTGGGCG25700.039.22062756
GGGGGTG28850.039.1842858
GCCGGGG16350.038.5309750
GCGTGAA44550.038.49493460
TCGGCGT27750.038.08896357
GTGGGGG32300.036.9493756
TATGCCG132400.036.50571447
TGGGCGT31950.035.9300857
CATCTCG160400.035.28262340
GCCGTCG46000.035.1511350
GTATGCC140250.035.08630846
GTCGGCG20000.034.99896656
ATGCCGT118300.034.88062348
CGGCGTG30400.034.42332558
CGTCGGC20450.034.22881755
CGTGAAA52100.033.65543461
Produced by FastQC (version 0.11.5)