FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n01_DS2228-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n01_DS2228-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6980928
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG974761.3963186556285927TruSeq Adapter, Index 9 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTG20100.041.6153558
GCGTGAA38250.037.60642660
GGCGTGA19400.036.8027459
TCGGCGT23200.036.20553257
TGCCGTC206300.035.69424449
TATGCCG220750.035.65664347
ATGCCGT218300.035.43155748
GTATGCC225050.035.1308746
GCCGTCT174300.034.6573350
CTCGTAT222400.034.4164243
GCTTGAA156500.034.37250560
GATCTCG226550.034.1412940
CCGTCTT179450.033.77972851
GCCGTCG40150.033.3860450
AGATCTC235050.032.90665439
CTGCTTG139850.032.88458
ATCTCGT235400.032.8131241
TCTCGTA234300.032.77298442
AAAAAGG156700.032.4971870
CGTATGC242300.032.48536345
Produced by FastQC (version 0.11.5)